Making Petri Dishes

hello everybody today we are going to be,making petri dishes with,nutrient agar in it to grow bacteria,what you're going to see it's pretty,easy,you can make jello you'll be all set you,can do this,the key to this though you want to keep,things clean i'm the world's worst,cleaning lady,i'm here in your home having a pretty,spectacular tuesday,so we're going to go through all the,steps hopefully it'll make sense and,you'll be able to make petri dishes if,you wanted to on your own,so let's get started,so the supplies i have been using the,same equipment,for well over 15 years i bought the,stuff on my own,and it's worked fairly well one of the,the items you would love to have is,what's called an autoclave,which is basically a pressure cooker but,they're very expensive so i'm going to,show you the the ins and outs of what i,do,to kind of get around that so the stuff,you're going to need there's quite a,list,you need a scale you need a hot plate,you're going to need nutrient agar,aluminum foil,sterile says on the bag sterile petri,dishes,a container to do some mixing in,some styrofoam cups,and your pressure cooker,with some other containers in them,so and water and lots of water okay so,that's what we're going to do i'm going,to run through the steps and we'll do a,couple different,angles from that so you can see how it,goes but to get this thing started i do,need,the heat going because it takes a while,to actually do this properly so that's,gonna heat up and i'm gonna let,this other stuff go down all right let's,do it,so i add some water to the bottom of the,pressure cooker,and now i figure out how much powder,agar i need,i will turn on my scale zero it with the,container on there,shaking the correct amount in this case,it was 11.5 grams for 500 milliliters,do the same thing with my other,container tear it so it reads zero put,11.5,into this container and now i'm going to,add the water i normally add hot water,to this it's like making hot cocoa,add the water to the powder it just,mixes easier,and now i transfer it over to my mixing,vessel,shake it up,and put it back into the container it,doesn't need to be exactly totally mixed,but you know a good mixing is good and,then i will cover it with foil,and i will do the same with the other,container i'll now load them into my,pressure cooker,which should be pretty hot you can see,the steam coming out of it and now i add,two styrofoam cups you'll see why i do,that later on,i'll now seal it up add the pressure,weight on the top,and let it go and it takes a while,and it 240. like we're done,our agar now is completely autoclaved or,cleaned and it's sterile just like our,dishes here,so i'll take all my dishes out and i'm,just going to explain the anatomy of,this dish,so when we pick up one of these dishes,and look at it it's got two parts it's,got the deep dish or chicago style,that's where the food is gonna go in and,then it's got the,thin crust or the lid where the agar is,not gonna go,so the deep dishes in my left hand the,lid is in my right hand,and don't open dishes like i just did,because it will contaminate,them you're going to want to open it,this way and i call it the pacman,technique to,minimize the amount of stuff or bacteria,that can get into this,dish so now let's actually fill them,what we're going to do now is fill our,petri dishes now while we fill our petri,dishes i am going to mask up,and be very conscious of,keeping the lid on the dishes pretty,much at all times the only time,the lid comes off is when i'm going to,bring in my agar i'm going to open up,just the end so a very little amount,is exposed and i'm going to open up my,dish in the way that i like to call the,pacman technique,i'm just going to lift up the top barely,and i'm just going to pour in the,agar off to the side of it i do not take,the,the lid all the way off because bacteria,is going to fall in possibly from the,air,and contaminate my dish again i want,this completely sterile i don't want,anything,in it except for my agar i do just want,to briefly discuss the,cups so if you saw that i put the full,size,styrofoam cup in there and it shrank,down into this and the other neat thing,is that it,changed its properties too this is,somewhat squishy and soft and this is,actually pretty brittle now um so that's,kind of,cool i think so that's that and you can,talk about pressure and volume and all,that good stuff,along with that but i'm not gonna do,that now,so my dishes are now set i'm going to,leave them here,let them turn solid and i don't want to,move them because it'll cause things to,slosh around in there and stick to the,sides of your container don't want to do,that,in the meantime i do have empty,containers that i do need to clean,so i'm going to clean those and one of,the things you might notice,on these dishes is that there is some,condensation or moisture on the lid,that's perfectly fine you don't want a,ton of condensation in there,but this haze that you see on the dishes,is just fine,we'

Growing Bacteria - Petri Dish

Growing Bacteria - Petri Dish

아,scl 기술 잡을 좋겠어요 같은거 a,캐주얼이 5에는 썸 cao 퍼플 카우,cow epdm 쉽게 많이 써 안,분위기죠 비추 이제 command,has over 워낙 부쩍 비교분석,아 b2 제시를 줄일 수는 봤죠 이거,아래 & 애써서 안 명 추이 너 급히,dh ht 아파 우려에 내 파워를,겠지 아 프렌즈 아아 그러니까 5m,월 선포했다 이유도 쓰고 왜 서울,seoul 에뮬 풀 잉어 교육 그래도,써 비보이 유지하고 체내 는 포트,rob 라비 onl 베쓰 에서 옷,어딨어 라고 on left 하기에,어버이의 4 usd 스킨을 스태그 답,내는데 측은 화이 가 있어 제 4,찍은 하기 위해서 주세요 는 생각할,oo 그러면 어느 다 떨어지면 투자와,owo 소변 내 소원 초 버스 d #,어리니까 없어 회수 id 알 수,있거든요 동안 암 때 니라 5분께,없는 내 매부리 요인 4화 있을게,어때요 옛날 아 이제 쓴 뒤 뜻으로,예 소개했어요 몰라요 mix 스크립트,선 티볼 저항 튜브 nano kid,바이트 서지수 바삐 밖엔 볼 수 있듯,모임을 나 따져 저 덴 히어 주께서,주방이 보입니다 어떠어떠한 야 이,책의 비슷해 이게 밑에 있듯이 10,위의 이동해 보거나 오븐 a 겐 그,after a week 그래서 아,10% 즉 교회의 있어서 그랬군 아,이제 가게 됐으니 쇼에 히어 앤드,스파 올 ces 쓰기 변하게 될 수만,나올 네 마음이 내 마음 nude,세이드 좀 모아 마이크 오게 됐으니까,왜냐면 없구나 없네 뭐 쏘았나 어느,폰에 쪼개고 a 시작될 끝 음악 쉬운,읍 엡손 가야 되면 쇼 쓸 수,있는거예요 시작이 아쉬움에 애써 어머,수수 괜찮으면 얘도 좀 그렇구,two 에 배들이 생긴다 좀 제가,어제도 제가 쓰더라 열어 보 납사,라식 ama10 my soul 같으냐,빙 토우 빨대 또 신 오딧세이 to,the q x 꼬 x y 로 넣으셔서,영어를 가봐야 알겠지요 예 번째 스사,갈까 마이클과 캐스 지니게 4 멀티캠,써 아 그랬어 in 돌이 on lan,연수원 와 의 세계 태그 ots,ceo 가 거야 라고 예수 밖에 없어,수익의 의도대로 봐야 돼 예언의,인천에 허리 차원이 작가이고 찍어 이,거야 이렇게 버너 일상적인 초아 2,차 세 류시원 요나 도와 올수도 불소,넓게 나왔어요,4 우직하게 됐어요 임 밤 바다 앞에,무시,answers,outer tube 3 doors,서화 버티고 있는 언덕으로 에버리스,하면서 점이 you,wont esc 키 보호 i,owe,rfid 로 해서 얻은 life to,인데 4 to 6 4,왜냐 됐소 왜 60명에 뭔지 킹의,탱크 거리 hi-fi 오디오 스피커는,9 schoolmaster 지키게,녹화 이렇게 엠카 원이 즐거 랩을,그게 꽤 대칭 을 통해서 썸 led,3일차 해주고 쓰고 이렇게 예술대학,교수 사기 3 7 3 예외의 스를,카보 이 아악 c 키 밥알이 개업식,전용 오아시스 대화에서 듯 이것들도,파악해 승인 비롯한다 서핑 tvn 더,얻어 창 있느냐의 굉장히 나미아 4,500 예수의 피의 테스티노 4대,섬인데 줄 거 리 이놈이 애벌,대체적으로 와요 돌아오면 축소 회생한,담,작은 것을 확인할 집앞 ct scan,스켈링 후 영업일 커야 합니다 홀,보안도 인해 보기 세이버 리 싸온,플라이워치 스테인은 법회 11s 썸,워서 관해서 유섭 ipc 는 봐도,절개나 uc 오니까 1y 본 줄,알지요 ep 익혀서 아직 오빠,뛰어들어 단일 기업 센스 알 거에요,으 르

How To Grow Mushrooms: Part One: Germinating Spores In Agar Petri Dishes

How To Grow Mushrooms: Part One: Germinating Spores In Agar Petri Dishes

what is up y'all this is the fruit,farmer coming back at you with another,picky fruits video and this one we're,going to be doing some multi-sport,germinations on heat treat dishes so,stay tuned,for those of you need some psychology,spore is released by a mature mushroom,fruit body right before it decays,allowing for the reproduction of the,parent mycelium and i'm going to show,you some samples of how cultivators,collect spores for long-term storage,in today's episode i'm primarily going,to be showing you how to use square,swaps spore swabs are sterile swabs that,are pretty much like giant q-tips that,have been used to take a sample of,spores from underneath the mushroom cap,rubbing it along the gills to collect,these very dark spore collections onto,the sterile cotton,another invaluable way that cultivators,have learned to collect spores is via,spore print,i'm going to show you,what a sport print looks like and then,we will actually use a smart print to,try to germinate some of the spores onto,petri dishes,the way you take a sport print is by,taking a mature mushroom and placing it,cap,side up,gills down onto a fresh piece of,aluminum oil,and over several hours,what you get is a very dark print like,this with pronounced skills and dark,pigmentation,i will not show you how to use a sport,print i've already taken a sterile blade,and attached it to my scalpel we will,very gently,scrape,at the sport print collecting some of,this horse and you don't need very much,here you can see some of the spores,already on the plate,i will take my petri dish,and scrape,the blade,back and forth,on my petri dish,so you can see some of the spores have,already come in contact with the,agar,and we'll repeat the process two more,times now we will do our second,and third,scrape,and inoculation onto our plate,and like i said before you don't need,very many spores to make this happen all,you really need are two spores to,germinate on the petri dish,and the media that we're using today,is light malt extract with a little bit,of food coloring,and the third,time there you have it there is our,first spore,trial,for germination,and,the variety that we were using for this,is ecuador,so now that we've successfully put,spores onto our petri dish we are going,to seal it up and the way that we do,this is with the shriek band that goes,around the petri dish,and it is heated with the heat gun,creating a tight seal that will prevent,anything from coming into our petri dish,while our spores germinate and now that,our pantries are securely wrapped we are,going to,name it with the variety and date,so now that we've used our sport print,and it's out of the way i'm going to,show you how we use our spore swabs,now you have to remember that spore,swabs are generally not sterile and,therefore might still contain bacteria,or contaminants,so we will generally use three or more,petri dishes to try to germinate our,spores just in case some of them fail,so the way we use them is we take the,swab,and just like before we will take it and,we will swab it across the surface of,the petri dish,and again not much is needed,we are just looking for two spores to,germinate,to call it a success,and also remember that once you are done,with the swap,they are generally good for many many,years,once you have a positive germination or,if you have a successful germination you,can always come back to these for more,genetic material so now that we've,straightened our swab across our petri,media i'm going to seal it up and i,don't know if you can tell but you can,see,the z-shaped or slightly,shaped,swab that i made and the strain that we,use for this petri,is called dancing type,so like before i'm going to seal them,date them and label them and put them,away,and watch them very closely for signs of,germination,up next we have another swab and this,one is,swp,same as the last,this one happens to have a longer stick,streak it across the medium,next up we have yeti cross with choco,very nice and dark,we can tape these back up as soon as,we're done with them,put them back in the storage,up next we have mac cross with eight,and a lot of these mushrooms i've never,actually seen myself in the flesh,so it's very exciting to be able to,have them and use them,and hopefully they provide,some very nice and vigorous growth,that we can isolate from,this one here,is a,kosumai,squat,and you'll notice that some of these,swabs will have plastic handles,others will have wooden handles,and they will be different sizes,and it's really,just up to the cultivator and what they,choose to do and what they favor doing,some cultivators will break the stick,to have a shorter,stem,others will keep the whole thing intact,let me know in the comments if you have,any,experience with swabs and what you're,used to and what you like,up next we have,a pe,now some of these swabs,will come from,mushroom tissue instead of mushroom,spores,some mushrooms,do not sporulate,but you can still take their,sample on a swab so

How to Grow Bacteria

How to Grow Bacteria

,كيف تزرع بكتيريا,الأدوات المحتاجة,ماء/جيلاتين/مسحات قطنية(نكاشات اذن)/طبق بتري من البلاستيك,يصب 10غ من الجيلاتين في 330مل من الماء,اثارة الحل حتى يتم حلها بالكامل,ضع المحلول في الميكرويف,ضع الموقت على 4دقائق,خذ المحلول خارج الميكرويف واجعله يبرد 3 دقائق,صب المحلول في الطبق البلاستيكي ثم اغلقه بغطاء,بعد ساعة المحلول سيصبح صلب,استخدم المسحات القطنية (نكاشات اذن) لتأخذ عينات البكتيريا,على سبيل المثال يمكنك مسح مقبض(يد) الباب بالمسحات القطنية(نكاشات الأذن),ارسم خط متعرج على الجيلاتين ببطئ واغلق الغطاء,استعمل المسحات القطنية اكثر لاخذ المزيد من العينات,ضع الطبق في صندوق مغلق لمدة اسبوع,هنا النتائج,لنلقي نظرة اقرب,نحن نستطيع وضع اليد على الوعاء,هنا النتائج,هل هو ممتع؟,شكرا على المشاهدة

Four Quadrant Streak - How to properly streak a Petri plate for isolated colonies

Four Quadrant Streak - How to properly streak a Petri plate for isolated colonies

In order to correctly treat an infection or  identify a contaminant microbiologists must  ,first isolate the target microbe from a sample or  specimen that often contains other microorganisms.  ,Streaking agar plates is an essential technique  used in the laboratory to isolate pure individual  ,colonies from a larger sample source. Before  beginning it is important to remember individual  ,microbial cells are not visible to the naked eye.   Avoid the tendency to over-inoculate the plate  ,a common mistake that will cause the plate to  be overloaded with colonies and result in a  ,confluence of growth. This is particularly critical  for certain specimen types such as stool samples  ,where isolation of single colonies is extremely  important. Also agar is similar in consistency  ,and firmness to jello, and can be prone to breaking  the gel matrix. The goal when inoculating plates is  ,to streak the surface gently and not to cut, tear,  or stab the gel matrix with the inoculating loop.  ,To prepare ensure the bench top is clean,  disinfected, and organized. Make sure the tools such  ,as loops and labeling pens are within easy reach.  Laboratory personnel should use good hand hygiene,  ,aseptic technique, and wear appropriate clothing  such as a lab coat and gloves. Ensure clothing  ,is tight fitting near open flames or sterilizing  equipment such as a Bunsen burner or incinerator.  ,Allow the plate to reach room temperature prior  to inoculation to avoid temperature shock to  ,cells which may affect recovery. Always label  plates on the bottom of the dish prior to use.  ,This is essential to identify the sample  after incubation and to ensure the plate  ,can be identified in the event the lid is lost,  dropped, or accidentally switched to another plate.  ,The purpose of the streak plate technique is to  reduce the number of cells from an area of high  ,concentration from the original sample and where  the streak begins to an area of low concentration  ,in the final streak. This is accomplished by  using a series of loop passes over the agar  ,called the four quadrant method. By the final  streak the number of cells on the loop should  ,be diluted and low enough that single cells  are dispersed and isolated on the agar surface.  ,After incubation these cells will grow into  individual pure isolated colonies. Thus one  ,viable cell will give rise to one single colony-forming unit known as a CFU.,To streak plates three types of tools can be used a non-sterile  metal loop which can be repeatedly sterilized by   ,heat between uses, a pre-sterilized disposable  plastic loop, or a sterile specimen swab.  ,These loops come in different sizes  and provide a calibrated volume  ,making it easier for the microbiologist to  determine the number of cells per milliliter  ,in a liquid sample. Loops are generally calibrated  to deliver either 10 microliters or 1 microliter.  ,Depending upon the testing site or sample either  a standard lab bench or biological safety cabinet  ,which protects the microbiologist may be used.  To sterilize a metal loop begin by flaming or  ,incinerating the loop. Once it grows red hot and is  sterilized allow the loop to cool in ambient air.  ,Do not blow on or wave the loop to speed up the  cooling process and do not touch the loop to any  ,contaminated surface prior to sampling. Cool  the hot loop by touching it to a sterile area  ,of the Petri plate. Depending upon the sample  type use the loop to collect a small number of  ,cells from confluent growth, obtain a loop full  of liquid sample, or touch the edge of a single  ,colony from a source plate. Remove the plate  lid and place it face down on the lab bench.  ,Pick up the plate in one hand and the metal  loop in the other hand. Streak the sample using a  ,sweeping motion from side to side across quadrant  1 approximately a quarter of the agar surface.  ,To prevent contamination avoid streaking  too close to the perimeter of the agar  ,the outside perimeter is where airborne  contaminants are most likely to be located.  ,Re-sterilize the loop and remember to let the  loop cool before continuing to the next quadrant.  ,You can quickly do this by stabbing the loop  into the agar. Be sure to avoid the streak  ,lines. Rotate the plate 90 degrees using a similar  sweeping motion, streak the loop into the quadrant  ,1 streak lines two to three times, and then proceed  to streak the remainder throughout quadrant 2  ,using about a quarter of the agar surface. If you  used a specimen swab for quadrant 1 now switch  ,to the metal loop for quadrants 2, 3, and 4.  Only overlap quadrant 1 two or three passes and  ,no more. The idea is to continue to reduce the  number of cells spread across each quadrant  ,so don't over saturate the loop. Re-sterilize  and cool the loop in the agar before continuing.   ,Rotate the plate another 90 degrees and spread the  cells from quadrant 2 into quadrant 3 using  ,a similar reduction strategy and a quarter of the  agar

Bacterial Isolation on Petri Dish - Biology Lab Techniques

Bacterial Isolation on Petri Dish - Biology Lab Techniques

Start by cleaning and sterilizing the workspace.,Spray once with 10% Milton,and wipe thoroughly using clean tissue paper.,Repeat twice, but with 70% ethanol.,A diluted 70% solution of ethanol is actually a more potent disinfecting agent than pure ethanol.,One of the goals of growing Bacteria on solid medium is to grow individual colonies made of a single clone.,Plates can also be used for short-term storage in the fridge up to 3 weeks.,Light up the Bunsen burner to establish a cone of sterility.,Hot air creates an upwards current that prevents airborne contaminants from falling onto your work.,Make sure you conduct all of your sterile work within that zone.,Spray your hands with 70% ethanol to sterilize their surface.,If you opt for wearing gloves proceed in the same manner.,Sterilize your loop in the flame.,The hottest part of the flame is located just above the tip of the blue cone.,Let your loop cool down.,To ensure proper cooling stabbed your loop in a clean part of the medium once or twice.,Pick up a single colony by touching it with your loop.,Streak a clean Petri dish using the quadrant method.,The goal is to reduce the bacterial load at every streak to separate single bacterial cells.,Do not let the loop go back into the previous quadrants for most of the original inoculum was deposited.,Here is a visualization of the streaky made using a marker.,Sterilize your loop using the flame watch out for projections due to boiling of remaining material on your loop.,Remember that you should wear protective eyewear whenever using the Bunsen burner.,Label the back of your plate using a permanent partner.,Use Parafilm to seal the Petri dish in order to avoid excessive evaporation during incubation.,Parafilm also lets gas through which allows aerobic respiration throughout the incubation period.,Incubate your plate upside down overnight.,Pick up your plates on the next morning and look for single colonies.,This is an overnight culture of Escherichia coli.,Thanks for watching, please subscribe to our channel for more science videos.

Using Bacteria As Paint

Using Bacteria As Paint

This artwork is alive.,Each one is made from fungi and bacteria.,Ew, gross, right?,But this art is being used,to change people's minds about microorganisms.,The person credited with pioneering the art,is Alexander Fleming.,If that name sounds familiar,,it's because he's the scientist,who discovered the antibiotic properties of penicillin.,The organization behind these particular artworks,is called Petri Dish Picasso,,and it's easy to see why.,They wanted to give people more access,to the interesting world of microbiology,with a hands-on approach.,Kids, especially, get a kick out of painting,with these little things called microbes.,Here's how you paint with bacteria.,Maria Peñil Cobo: Today, I'm gonna draw a dandelion,,and those are my brushes.,Narrator: Agar is a jellylike substance,made from red seaweeds,used to culture bacteria and fungus in labs.,It's also an ingredient in foods,like gummy bears and Jell-O.,It's sterilized into a liquid,at about 250 degrees Fahrenheit,,poured into a petri dish, and left to cool and solidify.,This is the canvas and the life support for the microbiome.,A microbiome is defined as the microorganisms,in a particular environment,(including the body or a part of the body).,In separate containers, the paint,,or microbes, must be grown.,A liquid broth of water and nutrients does the trick.,A popular ingredient for making agar art is E. coli.,Each organism has different nutritional needs,and can produce different colors.,A single cell of E. coli can grow,to over several billion cells overnight.,Using a few different tools, the cultured bacteria cells,are taken from their stew and gently painted onto the agar.,Peñil Cobo: And for the flowers,,I'm gonna use a different technique with a pipette.,Narrator: It's almost like painting with invisible ink,because the cells need to grow into mature colony clusters,for you to see them.,They're put in an incubator,at their optimal growing temperature.,E. coli prefers to be grown,at about 99 degrees Fahrenheit.,Depending on how fast the microbes replicate,,the incubation step can take anywhere,from 12 hours to several days.,Then it's time to see if your agar art,is in the league of Pablo Picasso,,or maybe Jackson Pollock.,Agar art doesn't last forever.,As the cells run out of nutrients,,they will begin to die.,Some agar artists try to preserve their work,by casting it in resin, but not Petri Dish Picasso.,As they say, all good things must come to an end.

Homemade Microbial Growth Medium for Petri Dishes

Homemade Microbial Growth Medium for Petri Dishes

growing microorganisms is sort of like,raising pets you have to give them the,right kind of food and grow them at a,temperature they like to be successful,you can actually do this in your own,house by making homemade Petri plates,containing a growth medium you can make,using water,Boyan cubes sugar and either gelatin or,agar agar now for the plates themselves,you can either use real petri dishes or,you can use Tupperware containers or,even muffin tins work if you see them in,a plastic baggie so to get started the,first thing you want to do is add your,water to something that you can either a,pan you can boil on the stove or a,microwavable container then add a,bullion cube and two teaspoons of sugar,now the sugar and the bullion cubes are,some of the food that we're feeding our,microorganisms finally you want to add,either one-and-a-half packages of,gelatin plain gelatin or a tablespoon of,agar agar now agar agar is made from,algae you can find it in it and the,Asian section of a number of grocery,stores the great thing about auger auger,is that unlike gelatin it won't melt at,high temperatures Agra auger also isn't,broken down by some microorganisms,gelatin plates can get broken down by,bacteria and actually sort of little,advice so if you can find it Agha Rogers,fantastic if not you can go with gelatin,that'll work just fine so I'm going to,take this mixture of microbe food over,to the microwave and alternately cook it,and stir it make sure an adult,supervises this part because the agar,agar gets very hot when your auger,agility has dissolved cover it loosely,with aluminum foil,let it cool for a few minutes then you,can start pouring your plates,remove the cover take your petri dishes,these are sections so I have to pour,three times and pour in about an eighth,to a quarter of an inch of growth medium,then to be sure to cover your plate and,set it down to cool and the gelatin or,augur will then Harden and you'll end up,with a jello like substance like I said,before you can also use Tupperware,Tupperware works perfectly fine for,plastic ware just pour in some growth,medium don't seal the top loosely cover,them or you can even use a metal muffin,tin,pour your medium in and then cover it,loosely or just put it right in a,plastic bag to cool when your plates are,cool you're ready to take your samples,take your plate if there's condensation,or water on the lid just wipe it off,with a paper towel grab a q-tip and,start sampling you can test different,surfaces around your house like,telephones or light switches or computer,keyboards take your q-tip you used to,take the sample label your plate take,the lid off and gently swab this q-tip,onto the plate you're putting microbes,on the plate now when your samples are,all done,take your plate put it on a counter top,out of the way and let your micro to,scroll after several days you should,start to see small white and yellow,bacterial colonies forming and if you,leave your plates for a while you might,see large fuzzy colorful fungal colonies,as well so junior microbiologist what,are you waiting for,make some plates and take some samples

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